Journal: Scientific Data

Article Title: Cytotoxic and Mechanical Properties of Resins 3D Printed with Low-Cost Hardware for C2C12 Biohybrid Actuators

doi: 10.1038/s41597-025-05738-7

Figure Lengend Snippet: Specimen samples for the ( A ) rigid resin samples as per ASTM D638 and ASTM D695 recommendations and for the ( B ) elastomeric resin samples as per ASTM D412 and ASTM D575 recommendations. (1) Tensile sample specifications. (2) Compression sample specifications. The drawings are not to scale with respect to each panel. (3) Actual samples of ( A.3 ) Phrozen AquaGray 8K and ( B.3 ) Formlabs Silicone 40A with IPA-post-treatment. Scale bars represent 10 mm. Refer to supplementary materials for detailed files. ( C ) Precurved cantilevered beam used in follow-up mechanical testing. (1) Isometric CAD drawing of the beam. Dead weights were hung by Kevlar threads to the dead weight attachment, and the fiducial markers were painted with acrylic paint to enhance contrast. (2) Centerline geometry of the beam centerline. This geometry was generated in MATLAB and exported as a 3D curve to SolidWorks. (3) Actual sample of a Phrozen AquaGray 8K beam. Scale bar is 40 mm.

Article Snippet: Formlabs Silicone 40A Formlabs , Various, including medical , ISO 10993-5:2009 ISO 10993-10:2021 ISO 10993-23:2021 , Elastomeric.

Techniques: Generated

Journal: Scientific Data

Article Title: Cytotoxic and Mechanical Properties of Resins 3D Printed with Low-Cost Hardware for C2C12 Biohybrid Actuators

doi: 10.1038/s41597-025-05738-7

Figure Lengend Snippet: Brightfield and fluorescence microscopy images of cells that were not exposed to any resin samples. These controls were used for the biocompatibility assessment of all resins except for the Formlabs Silicone 40A post-treated with IPA/BuOAc. ( A ) represent the blank (live cell) controls. ( B ) represent the positive (dead cell) controls, with ( B1 ) illustrating the cells prior to incubation with 70% ethanol. (1) are brightfield-only images of the cells prior to loading of any dye. (2) are brightfield images overlaid with fluorescence images of cells loaded only with calcein AM (green). (3) are brightfield images overlaid with fluorescence images of cells loaded only with ethidium homodimer-1 (red). All scale bars are 100 μ m.

Article Snippet: Formlabs Silicone 40A Formlabs , Various, including medical , ISO 10993-5:2009 ISO 10993-10:2021 ISO 10993-23:2021 , Elastomeric.

Techniques: Fluorescence, Microscopy, Incubation

Journal: Scientific Data

Article Title: Cytotoxic and Mechanical Properties of Resins 3D Printed with Low-Cost Hardware for C2C12 Biohybrid Actuators

doi: 10.1038/s41597-025-05738-7

Figure Lengend Snippet: Brightfield and fluorescence microscopy images of cells that were not exposed to any resin samples. These controls were used for the biocompatibility assessment of the Formlabs Silicone 40A post-treated with IPA/BuOAc. ( A ) represent the blank (live cell) controls. ( B ) represent the positive (dead cell) controls, with ( B1 ) illustrating the cells prior to incubation with 70% ethanol. (1) are brightfield-only images of the cells prior to loading of any dye. (2) are brightfield images overlaid with fluorescence images of cells loaded only with calcein AM (green). (3) are brightfield images overlaid with fluorescence images of cells loaded only with ethidium homodimer-1 (red). All scale bars are 100 μ m.

Article Snippet: Formlabs Silicone 40A Formlabs , Various, including medical , ISO 10993-5:2009 ISO 10993-10:2021 ISO 10993-23:2021 , Elastomeric.

Techniques: Fluorescence, Microscopy, Incubation

Journal: Scientific Data

Article Title: Cytotoxic and Mechanical Properties of Resins 3D Printed with Low-Cost Hardware for C2C12 Biohybrid Actuators

doi: 10.1038/s41597-025-05738-7

Figure Lengend Snippet: The CalAM:EthD-1 responses represented as boxplots of the ethanol/UV-sterilized rigid (dark blue-gray) and elastomeric (light blue-gray) resin samples, excluding the Formlabs Silicone 40A post-treated with IPA/BuOAc. Open circles (∘) represent individual data points. The red asterisks (*) denote outliers. The upper and lower boundaries of the box represent the first and third quartiles. Whiskers extend from the interquartile boxes to the minimum and maximum values, excluding outliers.

Article Snippet: Formlabs Silicone 40A Formlabs , Various, including medical , ISO 10993-5:2009 ISO 10993-10:2021 ISO 10993-23:2021 , Elastomeric.

Techniques:

Journal: Scientific Data

Article Title: Cytotoxic and Mechanical Properties of Resins 3D Printed with Low-Cost Hardware for C2C12 Biohybrid Actuators

doi: 10.1038/s41597-025-05738-7

Figure Lengend Snippet: The CalAM:EthD-1 responses represented as boxplots of the autoclaved rigid (dark pink) and elastomeric (light pink) resin samples, excluding the Formlabs Silicone 40A post-treated with IPA/BuOAc. Open circles (∘) represent individual data points. The red asterisks (*) denote outliers. The upper and lower boundaries of the box represent the first and third quartiles. Whiskers extend from the interquartile boxes to the minimum and maximum values, excluding outliers.

Article Snippet: Formlabs Silicone 40A Formlabs , Various, including medical , ISO 10993-5:2009 ISO 10993-10:2021 ISO 10993-23:2021 , Elastomeric.

Techniques:

Journal: Scientific Data

Article Title: Cytotoxic and Mechanical Properties of Resins 3D Printed with Low-Cost Hardware for C2C12 Biohybrid Actuators

doi: 10.1038/s41597-025-05738-7

Figure Lengend Snippet: The CalAM:EthD-1 responses represented as boxplots of the ethanol/UV-sterilized (dark blue-gray) and autoclaved (dark pink) Formlabs Silicone 40A post-treated with IPA/BuOAc samples and its associated PDMS reference standard samples. Open circles (∘) represent individual data points. None of the individual data points were outliers. The upper and lower boundaries of the box represent the first and third quartiles. Whiskers extend from the interquartile boxes to the minimum and maximum values, excluding outliers.

Article Snippet: Formlabs Silicone 40A Formlabs , Various, including medical , ISO 10993-5:2009 ISO 10993-10:2021 ISO 10993-23:2021 , Elastomeric.

Techniques: